Tissue Culture Deflasking & Acclimation Guide 🌱
What is Tissue Culture?
Tissue culture consists of taking a portion of a plant (e.g. the apex, a leaf or segment thereof, stem segment, meristem, embryo, node, seed, anther, etc.) and placing it in a sterile nutrient media (usually gelled, semi-solid) where one or many plants will regenerate.
Each order will receive a QR with the link to this guide.
Thank you for your purchase! These are the steps we personally use, with an almost 100% success rate when acclimating our tissue cultures.
1. Let your TC rest (48–72 hours)
Before opening the bag or container, let your tissue culture adjust to your growing environment for 48–72 hours. Simply place it where it will be growing over the next few months.
2. Prepare your workspace
Sterilize your work area and gather:
Distilled water
Tweezers
Soft toothbrush or paintbrush
Paper towels
Alcohol
Fungicide
Rooting hormone
Transplant vitamin
Your preferred substrate
A humidity dome/container (100% humidity for the first 6 weeks)
3. Remove all the agar
Using sterilized tweezers, gently remove the plant and rinse the roots thoroughly in distilled water. Use a soft brush to remove as much agar as possible, as leftover agar may encourage rot.
Don’t worry if a few leaves or roots fall off—this is completely normal. Remove any dead tissue before planting.
4. Fungicide dip
Soak the plant in a diluted fungicide solution for no longer than 10 minutes, following the manufacturer’s instructions. Rinse again with distilled water afterward.
5. Vitamin soak
Soak the plant in distilled water mixed with transplant vitamin for 10–15 minutes (up to 30 minutes if desired).
6. Air dry
Let the plant rest on a paper towel for 5–10 minutes to remove excess moisture. Don’t allow the roots to dry out completely.
7. Potting
We recommend:
Tree fern fiber
AAA sphagnum moss (pre-soak at least 3 hours with rooting hormone)
Coco/perlite mix
High quality propagation plugs
Pot into a small container, moisten the substrate (not soaking wet), and maintain 100% humidity for the first 6 weeks.
Provide bright indirect light or grow lights for 12–16 hours daily. (We strongly recommend buying a cheap light meter from Amazon, aiming for 500-800 Foot Candles (FC) is the sweet spot for growing healthy & vigorous TC’s)
8. Acclimation
New roots should begin forming within 2–3 weeks. Even if you see roots, keep humidity high.
After 6 weeks, gradually reduce humidity by opening the container for a few hours each day over the course of a week.
Use fertilizer at ½ strength for the first 4 months, then care for your plant as you would any established plant.
Questions?
We’re always happy to help! If you have any questions during the process, just send us a message. Good luck, and enjoy growing your new tissue culture! 💚🌱
Dosage & Dilution Rates for Acclimation Kit/Liquid Kit (Purchased with us):
DoseX (Rooting Hormone): 1 ml per gallon of water / .25 ml per liter.
ThriveX (Organic Fertilizer/Transplant Vitamin): 4 ml per gallon of water / 1 ml per liter.
FungiX (Fungicide): 5 ml per gallon of water / 1.25 ml per liter.
Each drop bottle contains 5 ml of each solution.
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